Journal: Journal of Cell Communication and Signaling

Article Title: Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes

doi: 10.1007/s12079-016-0323-0

Figure Lengend Snippet: Immunofluorescence images of Gja1, Gjb1 and Gjb2 in IL1-WT and IL1-KO cells after exposure to CNT-1 and CNT-2. IL1-WT and IL1-KO cells were plated on cover slips and were allowed to attach for 24 h before exposure to CNTs or dispersion media alone for 24 h. Cells were stained with the respective antibodies and fluorescent secondary antibodies were used to detect expression. Hoechst staining was used to visualize cell nuclei. a Representative images for Gja1. b Representative images for Gjb1. c Representative images for Gjb2. Arrows indicate interesting areas with hemichannels and gap junction channels when present between cells. For Gjb1 arrows also point to dividing cells having increased expression of Gjb1. Scale bar: 10 μm

Article Snippet: Antibodies used were as follows; Gja1 (rabbit connexin 43 polyclonal antibody, Cell Signaling Technology), Gjb1 (mouse anti-connexin-32 monoclonal antibody, Millipore), Gjb2 (goat anti-Gjb2 polyclonal antibody, Abcam), Sodium Potassium ATPase alpha 1 (mouse anti-NaK ATPase α1 monoclonal antibody, Abcam) and α-Tubulin (rabbit monoclonal antibody, Cell Signaling Technology).

Techniques: Immunofluorescence, Dispersion, Staining, Expressing

Journal: Journal of Cell Communication and Signaling

Article Title: Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes

doi: 10.1007/s12079-016-0323-0

Figure Lengend Snippet: Gja1 , Gjb1 and Gjb2 mRNA expression levels in IL1-WT or IL1-KO cells after exposure to dispersion media alone, CNT-1 or CNT-2 . a Gja1 mRNA expression levels investigated by qPCR after exposure to CNT-1 and CNT-2 for 24 h. b Gjb1 mRNA expression levels after exposure to the CNTs for 24 h. c Gjb2 mRNA expression levels after exposure to the CNTs for 24 h. Values represent the mean ± standard error (SE) of three independent experiments performed in triplicate. * P < 0.05 between IL1-WT and IL1-KO cells. # P < 0.05 between exposed IL1-WT or IL1-KO and their respective non-exposed controls

Article Snippet: Antibodies used were as follows; Gja1 (rabbit connexin 43 polyclonal antibody, Cell Signaling Technology), Gjb1 (mouse anti-connexin-32 monoclonal antibody, Millipore), Gjb2 (goat anti-Gjb2 polyclonal antibody, Abcam), Sodium Potassium ATPase alpha 1 (mouse anti-NaK ATPase α1 monoclonal antibody, Abcam) and α-Tubulin (rabbit monoclonal antibody, Cell Signaling Technology).

Techniques: Expressing, Dispersion

Journal: Journal of Cell Communication and Signaling

Article Title: Effects of carbon nanotubes on intercellular communication and involvement of IL-1 genes

doi: 10.1007/s12079-016-0323-0

Figure Lengend Snippet: Localization of Gja1, Gjb1 and Gjb2 protein after CNT exposure. Cytosolic ( c ) and integral membrane/membrane-associated ( m ) proteins were separated into two fractions. Western blot analysis was used to investigate the localization of Gja1, Gjb1 and Gjb2. Antibodies against NaK ATPase α1 and α-Tubulin were used to detect the membrane and cytosolic fractions, respectively. The images shown are representative of two independent experiments

Article Snippet: Antibodies used were as follows; Gja1 (rabbit connexin 43 polyclonal antibody, Cell Signaling Technology), Gjb1 (mouse anti-connexin-32 monoclonal antibody, Millipore), Gjb2 (goat anti-Gjb2 polyclonal antibody, Abcam), Sodium Potassium ATPase alpha 1 (mouse anti-NaK ATPase α1 monoclonal antibody, Abcam) and α-Tubulin (rabbit monoclonal antibody, Cell Signaling Technology).

Techniques: Membrane, Western Blot

Journal: eLife

Article Title: TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells

doi: 10.7554/eLife.64456

Figure Lengend Snippet: ( A ) Immunostaining of Cajal bands in teased P29 ventral roots with an antibody against βII spectrin. ( B ) Immunostaining of teased P29 ventral roots for ankyrinG (AnkG) (red, node) and Kv1.1 channels (white). Localization of Kv1.1 channels in the juxtamesaxon is indicated by open arrowheads. ( C ) Immunostaining of P28 sciatic nerves for zonula occludens-1 (ZO-1) (magenta) and βIV spectrin (green, node). ( D ) Immunostaining of P28 trigeminal nerves for connexin 32 (Cx32) (magenta) and neurofascin (NFasc) (green). ( E ) Immunostaining of P28 sciatic nerves for E-cadherin (E-cad) (red), myelin-associated glycoprotein (MAG) (blue), and NFasc (green). ( F ) Immunostaining of teased P28 sciatic nerves for MAG (green, incisure) and NFasc (magenta). cHet and cKO by Dhh-Cre ( A–F ). Scale bars, 5 μm ( A–F ).

Article Snippet: The following primary antibodies were used: mouse anti-actin (MilliporeSigma, C4), mouse anti-AnkB (UC Davis/NIH NeuroMab Facility, N105/17), mouse anti-AnkG (NeuroMab, N106/36), mouse anti-AnkG (NeuroMab, N106/65), mouse anti-βII spectrin (BD Biosciences, 42), chicken anti-βIV spectrin (a gift from Dr. Matthew N. Rasband), rabbit anti-βIV spectrin (a gift from Dr. Matthew N. Rasband, Baylor College of Medicine), mouse anti-Caspr (Mab275) , rabbit anti-Caspr , rabbit anti-Caspr (Abcam ab34151), rabbit anti-Caspr2 , goat anti-Cntn (R&D Systems, AF904), mouse anti-Cx32 (Thermo Fisher Scientific, 5F9A9), mouse anti-E-cadherin (BD Biosciences, 36), mouse anti-Gldn (Mab94) , rabbit anti-Gldn , rabbit anti-Krox20 (a gift from Dr.

Techniques: Immunostaining

Journal: eLife

Article Title: TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells

doi: 10.7554/eLife.64456

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used: mouse anti-actin (MilliporeSigma, C4), mouse anti-AnkB (UC Davis/NIH NeuroMab Facility, N105/17), mouse anti-AnkG (NeuroMab, N106/36), mouse anti-AnkG (NeuroMab, N106/65), mouse anti-βII spectrin (BD Biosciences, 42), chicken anti-βIV spectrin (a gift from Dr. Matthew N. Rasband), rabbit anti-βIV spectrin (a gift from Dr. Matthew N. Rasband, Baylor College of Medicine), mouse anti-Caspr (Mab275) , rabbit anti-Caspr , rabbit anti-Caspr (Abcam ab34151), rabbit anti-Caspr2 , goat anti-Cntn (R&D Systems, AF904), mouse anti-Cx32 (Thermo Fisher Scientific, 5F9A9), mouse anti-E-cadherin (BD Biosciences, 36), mouse anti-Gldn (Mab94) , rabbit anti-Gldn , rabbit anti-Krox20 (a gift from Dr.

Techniques: Staining

Journal: Medicina Oral, Patología Oral y Cirugía Bucal

Article Title: All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

doi: 10.4317/medoral.18693

Figure Lengend Snippet: Western blot and immunofluorescence analysis of Cx32, Cx43 and Cx45 protein expression in OSCC cells after ATRA treatment. The expressions of Cx32 (A) and Cx43 (B) significantly decreased in SCC9 and Tca8113 cells as compared with human normal oral epithelial (NOE) cells. After ATRA treatment for 48h, the protein expressions of Cx32 (A) and Cx43 (B) increased in both SCC9 and Tca8113 cells. (C) Compared with human normal oral epithelial (NOE) cells, the protein expression of Cx45 also decreased in SCC9 and Tca8113 cells. However, ATRA treatment did not changed the protein level of Cx45 in SCC9 and Tca8113 cells. (D)ATRA treatment significantly increased the abundance of Cx32 and Cx43 protein and improved localization of fluorescent spots on the plasma membrane of treated SCC9 and Tca8113 cells, compared with those of controls, where the fluorescence appears scattered in the cytoplasm. #P<0.01 relative to normal oral epithelial cells; *P<0.05 relative to ethanol control. Bar: 20μМ.

Article Snippet: Cells were incubated overnight at 4°C in 1:100 mouse anti-connexin 43 (Invitrogen) or 1:50 mouse anti-Cx32 antibody (Invitrogen).

Techniques: Western Blot, Immunofluorescence, Expressing, Fluorescence

Journal:

Article Title: Hepatocyte nuclear factor 4? orchestrates expression of cell adhesion proteins during the epithelial transformation of the developing liver

doi: 10.1073/pnas.0600246103

Figure Lengend Snippet: HNF4α is required for expression of cell junction and adhesion proteins in fetal mouse liver. (A) Immunoblots revealed a loss of or reduction in expression of proteins required for the formation of adherens junctions [E-cadherin (E-CAD)], tight junctions (CLDN1, F11R, and OCLN), and desmosomes (DSC2) in 18.5-dpc fetal livers lacking HNF4α (Hnf4aloxP/loxP;AlfpCre) compared with control livers (Hnf4aloxP/+;AlfpCre). β-Actin (ACTB) was used as a loading control. (B) Confocal microscopy demonstrated that the expression and localization of the tight junction protein CLDN1 and GJB1 (also known as connexin 32) were disrupted in 18.5-dpc fetal livers lacking HNF4α (Hnf4aloxP/loxP;AlfpCre) compared with control livers (Hnf4aloxP/+;AlfpCre).

Article Snippet: Antibodies against the following proteins were used: claudin-1 (rabbit polyclonal; Zymed; 1:100), connexin 32 (rabbit polyclonal; Zymed; 1:200), ZO1 (rabbit polyclonal; Zymed; 1:200), and Alexa-Fluor 488 goat anti-rabbit (Invitrogen; 1:500).

Techniques: Expressing, Western Blot, Confocal Microscopy

Journal:

Article Title: High-resolution proteomic mapping in the vertebrate central nervous system: Close proximity of connexin35 to NMDA glutamate receptor clusters and co-localization of connexin36 with immunoreactivity for zonula occludens protein-1 (ZO-1)

doi: 10.1023/B:NEUR.0000029653.34094.0b

Figure Lengend Snippet: Primary antibodies used in this study, the generating species, protein target, target amino acid sequence (if available) and species reactivities, and private vs. commercial sources.

Article Snippet: Anti-Cx32 (mouse monoclonal MAB3069; Chemicon) was used to label adult rat liver.

Techniques: Sequencing

Journal:

Article Title: High-resolution proteomic mapping in the vertebrate central nervous system: Close proximity of connexin35 to NMDA glutamate receptor clusters and co-localization of connexin36 with immunoreactivity for zonula occludens protein-1 (ZO-1)

doi: 10.1023/B:NEUR.0000029653.34094.0b

Figure Lengend Snippet: Simultaneous labeling of Cx32 in gap junctions and ZO-1 in tight junctions of rat liver, and comparison with ZO-1 labeling in tight junctions of capillary in adult rat retina. (A) Stereoscopic imaging (left pair) and reverse stereoscopic imaging (right pair) reveals that Cx32 labeling (12 nm gold) is directly associated with gap junctions (white arrowheads), whereas labeling for ZO-1 (18 nm beads) is associated exclusively with tight junctions strands (black arrowheads). (B) Portions of five capillary endothelial cells linked by tight junctions (arrow), each immunogold labeled for ZO-1 (12 nm gold beads). Asterisk (*) denotes capillary lumen. (C) Stereoscopic and reverse stereoscopic images of tight junctions (arrows), with ZO-1 labeling beneath the replica.

Article Snippet: Anti-Cx32 (mouse monoclonal MAB3069; Chemicon) was used to label adult rat liver.

Techniques: Labeling, Imaging